Allicin up-regulates cellular glutathione level in vascular endothelial cells.

BACKGROUND: Allicin in garlic is the primary active compound known to rapidly interact with free thiols. AIMS OF THE STUDY: To examine the effect of allicin on gene expression and glutathione cellular level in vascular endothelial cells. METHODS: Cultured endothelial cells were exposed to allicin; mRNA was prepared and subjected to Micro-array and Real-Time PCR. Glutathione cellular level was determined on cell lysates. RESULTS: Micro-array analysis demonstrated allicin-induced up- and down-regulation of 116 and 100 genes, respectively. Up-regulated genes included the phase II detoxifying enzymes thioredoxin reductase 1 and 2, heme oxygenase-1 and glutamate cysteine lygaze modifier subunit, the rate limiting enzyme in glutathione biosynthesis. Endothelial cells exposed to allicin and its derivatives containing glutathione or cysteine residues increased cellular glutathione. Allicin increased the glutathione level in a concentration and time-dependent manner up to 8-fold at a concentration of 10-20 microM after 28 h exposure. Furthermore, allicin derivative-treated cultures demonstrated a 50% decrease in tBuOOH cytotoxicity. CONCLUSIONS: These results may suggest a putative role for allicin and its derivatives in preventing reactive oxygen species damage by up-regulating the phase II detoxifying enzymes and increasing the cellular glutathione level. PMID: 19048328 [PubMed - indexed for MEDLINE]

Eur J Nutr. 2009 Mar;48(2):67-74. Epub 2008 Dec 1. Allicin up-regulates cellular glutathione level in vascular endothelial cells. Horev-Azaria L, Eliav S, Izigov N, Pri-Chen S, Mirelman D, Miron T, Rabinkov A, Wilchek M, Jacob-Hirsch J, Amariglio N, Savion N. Goldschleger Eye Research Institute, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.

Stabilized Allicin has been shown to increase Glutathione (6SH)(article 2)

Clinical Research suggests that your daily diet could benefit from adding a high quality nutraceutical designed to help maintain a healthy natural defense system. The use of stabilized allicin, the primary active agent generated by garlic, for general well being is on the increase and it’s role as an antioxidant has been widely investigated. Stabilized Allicin has been shown to increase Glutathione (6SH).

British scientists have developed a proprietary process through which the naturally occurring allicin in garlic is extracted, stabilized and concentrated. The end result is the extremely potent and effective product: Allimed ®. Stabilized allicin is the result of a patented process which produces purified, living-source, completely stable allicin-for the first time in history. Allicin is not garlic - but the fully potent, active factor in concentrated form, never before available. It has broad spectrum, anti-infective properties coupled with strong, immune boosting and immunomodulating capacity. This makes stabilized allicin a highly significant antimicrobial clinical agent, especially in today’s world of widespread infections and with the creation of new forms of multi drug resistant super bugs that have emerged due to the overuse of traditional antibiotics.

At last, genuine stabilized allicin provides controlled, gentle, yet exquisitely through biofilm degradation and destruction, critical to overcoming many chronic illnesses, often recalcitrant to many other means. Many doctors have seen miracles for chronic acne, sinusitis, intestinal dysbiosis, prostate bacterial infections, MRSA, H-Pylori, Parasites, Flukes, Pseudomonas, and severe biofilm congestion in many other organs and glands.

New stunning worldwide research in the past decade has linked the majority of chronic degenerative diseases, once believed to be due to poor lifestyle choices, genetics or other factors, instead to hidden infections, previously undiagnosed until the recent advent of more sophisticated techniques (such as PCR- polymerase chain technology). For example, kidney stones have now been linked to nanobacterial infection. Over 80% of heart disease has been linked to infection by Chlamydia pneumoniae.